Designing projects on gene expression and epigenetics

Are you interested in learning more about epigenetics, the chromatin landscape and gene expression and regulation, but unsure of which methodology to implement and when?

ABSTRACT:

How to Select the Appropriate Assay for Disease Research – From Exploratory ATAC-Seq to Targeted ChIP-Seq, CUT&Tag, CUT&RUN
Presenter:
Dr. Rwik Sen, Ph.D. – Active Motif Field Application Scientist
Summary:
Cutting-edge methodologies to study epigenetic regulations of development, health and disease have reshaped research. The methodologies are broadly categorized into exploratory/discovery e.g. profiling chromatin accessibility and interactions, and targeted e.g. detecting interactions between accessible chromatin and transcription factors, histone modifications, other proteins. A popular exploratory method is ATAC-Seq (Assay for Transposase Accessible Chromatin and Sequencing) which provides genome-wide maps of chromatin accessibility. If some of the accessible regions bioinformatically correspond to loci associated with transcription e.g. gene promoters, enhancers, transcription factor binding motifs, then the next steps are to select an appropriate targeted investigation method. Three such popular methods are ChIP-Seq (Chromatin Immuno-Precipitation), CUT&Tag (Cleavage Under Targets and Tagmentation), and CUT&RUN (Release Under Nuclease). They reveal genome-wide regions of interaction between accessible chromatin/DNA and entities of interest, e.g. transcription factors, histone modifications, other proteins. Overall, the above methods need to be carefully selected depending on your goals and samples. Hence, this presentation explores the workflow, deliverables, advantages of these assays, and case studies pertaining to NGS-based studies and oncology.

Speaker Bio:

Rwik Sen obtained his Ph.D. from Southern Illinois University Carbondale, and attended postdoctoral training at the University of Colorado and also received a postdoctoral fellowship from American Heart Association. His research has focused on epigenetics, transcription, DNA repair, and developmental biology. Rwik has over 25 peer-reviewed publications including book chapters. He is currently at Active Motif in California, the industry lead in epigenetics-focused biotechnology company that offers cutting-edge epigenetics portfolio comprising of products, services, instrumentation, and educational resources.

This seminar is brought to you by Active Motif, the industry leader in epigenetics research tools and services, in collaboration with Dr. Yadira Soto-Feliciano, PhD Howard S.(1953) and Linda B. Stern Career Development Professor, Assistant Professor of Biology –Koch Institute for Integrative Cancer Research at MIT AND The Genomics and Informatics Facilities Core of MIT. Lunch will be provided.

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