Active Motif CUT&RUN Hand-On workshop at MIT

CUT&RUN (Cleavage Under Targets & Release Using Nuclease)

This epigenetic method is used to investigate the genome-wide distribution of various chromatin-associated proteins and their modifications. CUT&RUN is a derivative of chromatin immunocleavage (ChIC). CUT&RUN is similar to chromatin immunoprecipitation (ChIP), in that it utilizes an antibody to target chromatin-associated marks and proteins, but requires less sample material and less sequencing depths than ChIP.

Unlike ChIP, CUT&RUN does not require crosslinking of the protein to the DNA, eliminating the introduction of artifacts. CUT&RUN is a valuable tool for studying chromatin-associated proteins such as transcription factors or histone modifications genome-wide.

Ideal for: Post-docs, RAs or grad students new to epigenetics research or studying the role of transcription factors and other DNA binding proteins on gene regulation

Workshop Objectives:

1. Hands on lab training with Active Motif’s CUT&RUN Kit
2. In-depth protocol review highlighting challenging steps and how to overcome them, QC steps required, and trouble shooting scenarios.
3. Data analysis training using CUT&RUN raw example data set / FASTQ files

Agenda:

• January 28th 1:30pm - 4:30pm Introduction lecture, wet lab using real samples
• January 29th 9:00am - 1:00pm: Wet lab continued from day 1(through library prep) using real samples, raw data analysis bioinformatics presentation and Q&A with example data set.

Required: Must bring your own pipets and tips and plan to start at 9am

Spaces are limilited therefore this workshop is by invitation only

Questions? Contact Cathy Stickney, Active Motif Local Sr. Account Manager: Email: [email protected], Cell: 603-339-6596

Wokshop by Rwik Sen, Ph.D, Active Motif Sr. Field Application Specialist and Product Manager Email: [email protected]

Want to learn more:

https://www.activemotif.com/catalog/1373/chic-cut-run-kit

Manual:

https://www.activemotif.com/documents/2263.pdf

Host or Publisher Active Motif