Genome Editing with CRISPR-Cas9

Four-part course:

Saturday, February 8, 2:00pm-6:00pm EDT

Sunday, February 9, 2:00pm-6:00pm EDT

Monday, February 10, 6:00pm - 9:00pm EDT

Sunday, February 16, 2:00pm-6:00pm EDT

CRISPR/Cas9 is one of the most transformative recent developments in biotechnology, radically simplifying the process of editing genomes and increasing its accessibility. Want to learn how to do hands-on genome editing? This is an intensive laboratory class with limited space.

In this class you will:

• Learn how to culture and work with Saccharomyces cerevisiae (brewers yeast)
• Assemble a plasmid encoding a CRISPR/Cas9-mediated gene deletion
• Generate a repair template encoding a fluorescent protein that will be inserted into the yeast genome
• Use the constructs you produce to transform yeast and confirm the results by DNA sequence analysis
• Discuss emerging technologies and applications of CRISPR in other organisms

Prerequisites:

• You must be familiar with molecular biology lab techniques such as pipetting, gel electrophoresis, use of restriction enzymes and PCR (Genspace Biohacker Boot Camp or equivalent lab experience)

COVID-19 Safety Notice:

If you are feeling unwell, suspect that you have been exposed to COVID-19 or have tested positive in the past 7 days, please do not attend and let us know ASAP ([email protected]). If you cancel after our 7-day policy, we cannot refund your ticket, but we can exchange and offer credits toward future classes. If you have signed up for Biohacker Boot Camp, we will automatically transfer your registration to the next month’s dates unless you tell us otherwise.

Brendan Camellato (he/him) is a postdoctoral research fellow at Memorial Sloan Kettering Cancer Center. He was born and raised outside of Ottawa, Canada, and attended the University of Ottawa where he completed a BSc in Biomedical Sciences and an M.Sc. in Cellular and Molecular Medicine. He currently works on developing tools for synthetic biology, genome engineering, and molecular recording, and uses them to study developmental and cancer biology. Brendan is very excited about the possibilities that biotechnology can bring to the world, and about helping others better understand these possibilities too. He is also very passionate about hockey and rock music, and when not in the lab can often be found on a hockey rink or behind a drum kit.

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